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| Characterization of Silk Fibroin/Hyaluronic Acid Blend Film Cross-linked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide |
| Shu-Qin Yan, Qiang Zhang, Jian-Nan Wang, Ming-Zhong Li |
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Abstract Silk fibroin (SF) / hyaluronic acid (HA) blend films were prepared using 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) as crosslinking agent and N-hydroxysuccinimide (NHS), 2-morpholinoethanesulfonic acid (MES) as assistant agents. The physico-chemical properties of the blend films were examined and analyzed. The FTIR shows that HA interferes with SF to form crystal structure, whereas EDC induced SF to form Silk I. Compared to the uncross-linked blend films, the EDC cross-linked blend films show an obvious increase of elongation at break, a decrease of tensile strength and Young's modulus, the flexibility of the films improved significantly. L929 cells grow well on SF/HA blend films, demonstrating that the blend films cross-linked by EDC have no significant toxicity.
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| Cite this article: |
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Shu-Qin Yan,Qiang Zhang,Jian-Nan Wang, et al. Characterization of Silk Fibroin/Hyaluronic Acid Blend Film Cross-linked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide[J]. Journal of Fiber Bioengineering and Informatics, 2010, 3(2): 62-67.
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2010, Vol. 3 
