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| Fabrication and Characterization of Spongy Porous Silk Fibroin Materials Crosslinked by Genipin
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| Biomedical Materials and Engineering Research Center, Wuhan University of Technology Wuhan 430070, China
National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering Soochow University, Suzhou 215123, China
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Abstract The porous Silk Fibroin (SF) scaffolds crosslinked by Genipin (GP) were prepared by freeze-drying. The
different amount of GP were added in different concentrations of SF solutions. Compared to the uncross-
linked porous SF scaffolds, the GP crosslinked scaffolds were insoluble and showed uniform pores and
porosity. The SF scaffolds, with average pore size 39-167 m and porosity 49-71%, could be obtained
by changing concentrations of fibroin solution. When the concentration of fibroin increased, the pore size
diminished. The fourier transform-infrared (FT-IR) results revealed that GP could react with the -NH2
groups on the side chains of SF macromolecules to form inter- and intro-molecular covalent bonds. The
FT-IR, X-ray Diffraction (XRD) and Differential Scanning Calorimetry (DSC) results also showed that
the crystallinity of SF scaffold crosslinked by GP was increased slightly while the thermal stability was
improved. This new fabrication way provided the basis theory for producing scaffolds with reasonable
structure.
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| Fund:Project supported by the National Nature Science Foundation of China (30970714), the College Natural Science
Research Project of Jiangsu Province (12KJA430003), the Key Program in Medical Science Research of Military
(No. BWS11C061) and the Priority Academic Program Development of Jiangsu Higher Education Institutions.
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| Cite this article: |
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Yiyu Wang,Xinyu Wang,Junhua Zhang, et al. Fabrication and Characterization of Spongy Porous Silk Fibroin Materials Crosslinked by Genipin
[J]. Journal of Fiber Bioengineering and Informatics, 2014, 7(4): 469-478.
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2014, Vol. 7 
